6pns

Electron Microscopy
3.7Å resolution

In situ structure of BTV RNA-dependent RNA polymerase in BTV virion

Released:
Source organism: Bluetongue virus 1
Primary publication:
In situ structures of RNA-dependent RNA polymerase inside bluetongue virus before and after uncoating.
Proc Natl Acad Sci U S A 116 16535-16540 (2019)
PMID: 31350350
Related structures: EMD-20398

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-172772 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 1302 amino acids
Theoretical weight: 149.93 KDa
Source organism: Bluetongue virus 1
UniProt:
  • Canonical: W0G557 (Residues: 1-1302; Coverage: 100%)
Sequence domains: Orbivirus RNA-dependent RNA polymerase (VP1)
Core protein VP3 Chains: B, C, D, E, F, G, H, I, J, K
Molecule details ›
Chains: B, C, D, E, F, G, H, I, J, K
Length: 901 amino acids
Theoretical weight: 103.41 KDa
Source organism: Bluetongue virus 1
UniProt:
  • Canonical: Q1AE73 (Residues: 1-901; Coverage: 100%)
Gene name: VP3
Sequence domains: Orbivirus VP3 (T2) protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-20398