› 6pnx

X-ray diffraction
2.2Å resolution

Crystal Structure of an Asymmetric Dimer of FGF Receptor 3 Kinases Trapped in A-loop Tyrosine Transphosphorylation Reaction

Released:
DOI: 10.2210/pdb6pnx/pdb
PDB entry 6pnx coloured by chain, front view.
PDB entry 6pnx coloured by chain, side view.
PDB entry 6pnx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for receptor tyrosine kinase A-loop tyrosine transphosphorylation.
Chen L, Marsiglia WM, Chen H, Katigbak J, Erdjument-Bromage H, Kemble DJ, Fu L, Ma J, Sun G, Zhang Y, Liang G, Neubert TA, Li X, Traaseth NJ, Mohammadi M
Nat Chem Biol 16 267-277 (2020)
PMID: 31959966

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22607 (Residues: 451-759; Coverage: 39%)
Gene names: FGFR3, JTK4
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

2 bound ligands:
Ligand ACP 2 x ACP
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 54.686Å b: 93.253Å c: 75.49Å
α: 90° β: 103.06° γ: 90°
R-values:
R R work R free
0.195 0.193 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Fibroblast growth factor signalling in osteoarthritis and cartilage repair.
Xie et al. (2020)
3 more

8 mentions without citation

Fibroblast growth factor receptor fusions in cancer: opportunities and challenges.
Chen et al. (2021)
7 more