› 6pom

Electron Microscopy
4.9Å resolution

Cryo-EM structure of the full-length Bacillus subtilis glyQS T-box riboswitch in complex with tRNA-Gly

Released:
DOI: 10.2210/pdb6pom/pdb
PDB entry 6pom coloured by chain, front view.
PDB entry 6pom coloured by chain, side view.
PDB entry 6pom coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis of amino acid surveillance by higher-order tRNA-mRNA interactions.
Li S, Su Z, Lehmann J, Stamatopoulou V, Giarimoglou N, Henderson FE, Fan L, Pintilie GD, Zhang K, Chen M, Ludtke SJ, Wang YX, Stathopoulos C, Chiu W, Zhang J
Nat Struct Mol Biol 26 1094-1105 (2019)
PMID: 31740854
Related structures: EMD-20416

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195828 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
T-box GlyQS leader (155-MER) Chain: A
Molecule details ›
Chain: A
Length: 169 nucleotides
Theoretical weight: 54.73 KDa
Source organism: Bacillus subtilis
Expression system: in vitro transcription vector pT7-TP(deltai)
tRNAGly (75-MER) Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Bacillus subtilis
Expression system: in vitro transcription vector pT7-TP(deltai)
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-20416
Expression system: in vitro transcription vector pT7-TP(deltai)

Quick links

Citations

11 review citations

Cryo-EM advances in RNA structure determination.
Ma et al. (2022)
10 more

8 mentions without citation

Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.
Zhang J. (2020)
7 more