› 6ppg

X-ray diffraction
2.75Å resolution

Crystal structure of IL17FF bound to Fab fragments of MCAF5352A

Released:
DOI: 10.2210/pdb6ppg/pdb
Model geometry
Fit model/data
PDB entry 6ppg coloured by chain, front view.
PDB entry 6ppg coloured by chain, side view.
PDB entry 6ppg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dissecting the molecular basis of high viscosity of monospecific and bispecific IgG antibodies.
Tilegenova C, Izadi S, Yin J, Huang CS, Wu J, Ellerman D, Hymowitz SG, Walters B, Salisbury C, Carter PJ
OpenAccess logo MAbs 12 1692764 (2020)
PMID: 31779513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-17F Chains: F, G
Molecule details ›
Chains: F, G
Length: 133 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96PD4 (Residues: 31-163; Coverage: 100%)
Gene name: IL17F
Sequence domains: Interleukin-17
Fab MCAF5352A light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 216 amino acids
Theoretical weight: 23.53 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Fab MCAF5352A heavy chain Chains: B, H

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand CD 53 x CD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 103.82Å b: 103.82Å c: 243.327Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.224 0.275
Expression systems:
  • Spodoptera frugiperda
  • Cricetulus griseus

Quick links

Citations

6 review citations

Progress and challenges for the machine learning-based design of fit-for-purpose monoclonal antibodies.
Akbar et al. (2022)
5 more

2 mentions without citation

Dissecting the molecular basis of high viscosity of monospecific and bispecific IgG antibodies.
Tilegenova et al. (2020)
1 more