› 6ppn

X-ray diffraction
1.91Å resolution

Structure of S. pombe Lsm2-8 with unprocessed U6 snRNA

Released:
DOI: 10.2210/pdb6ppn/pdb
PDB entry 6ppn coloured by chain, front view.
PDB entry 6ppn coloured by chain, side view.
PDB entry 6ppn coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes.
Montemayor EJ, Virta JM, Hayes SM, Nomura Y, Brow DA, Butcher SE
RNA 26 1400-1413 (2020)
PMID: 32518066

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-126961 (preferred)
Entry contents:
7 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
U6 snRNA-associated Sm-like protein LSm2 Chains: B, J
Molecule details ›
Chains: B, J
Length: 96 amino acids
Theoretical weight: 11 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O94408 (Residues: 1-96; Coverage: 100%)
Gene names: SPCC1620.01c, lsm2
Sequence domains: LSM domain
Probable U6 snRNA-associated Sm-like protein LSm3 Chains: C, K
Molecule details ›
Chains: C, K
Length: 95 amino acids
Theoretical weight: 10.82 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y7M4 (Residues: 1-93; Coverage: 100%)
Gene names: SPBC9B6.05c, lsm3
Sequence domains: LSM domain
Probable U6 snRNA-associated Sm-like protein LSm4 Chains: D, L
Molecule details ›
Chains: D, L
Length: 129 amino acids
Theoretical weight: 15.01 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O14352 (Residues: 1-121; Coverage: 100%)
Gene names: SPBC30D10.06, lsm4
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm5 Chains: E, M
Molecule details ›
Chains: E, M
Length: 80 amino acids
Theoretical weight: 8.86 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O42978 (Residues: 1-80; Coverage: 100%)
Gene names: SPBC20F10.09, lsm5
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm6 Chains: F, N
Molecule details ›
Chains: F, N
Length: 77 amino acids
Theoretical weight: 8.49 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UUI1 (Residues: 1-75; Coverage: 100%)
Gene names: SPAC2F3.17c, lsm6
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm7 Chains: G, O
Molecule details ›
Chains: G, O
Length: 119 amino acids
Theoretical weight: 13.32 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O74499 (Residues: 1-113; Coverage: 100%)
Gene names: SPCC285.12, lsm7
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm8 Chains: H, P
Molecule details ›
Chains: H, P
Length: 94 amino acids
Theoretical weight: 10.57 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O74483 (Residues: 1-94; Coverage: 100%)
Gene names: SPCC1840.10, lsm8
Sequence domains: LSM domain
Mimic of unprocessed U6 snRNA Chains: A, I
Molecule details ›
Chains: A, I
Length: 6 nucleotides
Theoretical weight: 1.79 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Unit cell:
a: 70.28Å b: 135.39Å c: 168.02Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.
Passmore et al. (2022)
3 more

1 mention without citation

Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes.
Montemayor et al. (2020)