› 6pql

X-ray diffraction
2.65Å resolution

SBP RafE in complex with raffinose

Released:
DOI: 10.2210/pdb6pql/pdb
PDB entry 6pql coloured by chain, front view.
PDB entry 6pql coloured by chain, side view.
PDB entry 6pql coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: The raffinose-family oligosaccharide utilization system.
Hobbs JK, Meier EPW, Pluvinage B, Mey MA, Boraston AB
J Biol Chem 294 17197-17208 (2019)
PMID: 31591266

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar ABC transporter, sugar-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 416 amino acids
Theoretical weight: 46.44 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2URJ7 (Residues: 24-419; Coverage: 95%)
Gene names: SP_1897, rafE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU, GLA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P21
Unit cell:
a: 48.862Å b: 145.823Å c: 118.677Å
α: 90° β: 92.64° γ: 90°
R-values:
R R work R free
0.25 0.248 0.293
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

α-Galactosidase and Sucrose-Kinase Relationships in a Bi-functional AgaSK Enzyme Produced by the Human Gut Symbiont Ruminococcus gnavus E1.
Lafond et al. (2020)
3 more