› 6pqu

Electron Microscopy
3.3Å resolution

Cryo-EM structure of HzTransib/nicked TIR substrate DNA pre-reaction complex (PRC)

Released:
DOI: 10.2210/pdb6pqu/pdb
PDB entry 6pqu coloured by chain, front view.
PDB entry 6pqu coloured by chain, side view.
PDB entry 6pqu coloured by chain, top view.
Source organism: Helicoverpa zea
Primary publication:
Structures of a RAG-like transposase during cut-and-paste transposition.
Liu C, Yang Y, Schatz DG
Nature 575 540-544 (2019)
PMID: 31723264
Related structures: EMD-20453

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109136 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Putative DNA-mediated transposase Chains: A, E
Molecule details ›
Chains: A, E
Length: 497 amino acids
Theoretical weight: 56.58 KDa
Source organism: Helicoverpa zea
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: B0F0C5 (Residues: 17-507; Coverage: 93%)
DNA (5'-D(P*AP*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*T)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Helicoverpa zea
Expression system: Not provided
DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Helicoverpa zea
Expression system: Not provided
TIR substrate DNA transferred strand Chains: D, H
Molecule details ›
Chains: D, H
Length: 32 nucleotides
Theoretical weight: 9.79 KDa
Source organism: Helicoverpa zea
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ZN 2 x ZN
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-20453
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

7 review citations

Structural insights into the evolution of the RAG recombinase.
Liu et al. (2022)
6 more

2 mentions without citation

Inner workings of RAG recombinase and its specialization for adaptive immunity.
Chen et al. (2021)
1 more