› 6pqy

Electron Microscopy
4.2Å resolution

Cryo-EM structure of HzTransib/TIR DNA transposon end complex (TEC)

Released:
DOI: 10.2210/pdb6pqy/pdb
PDB entry 6pqy coloured by chain, front view.
PDB entry 6pqy coloured by chain, side view.
PDB entry 6pqy coloured by chain, top view.
Source organism: Helicoverpa zea
Primary publication:
Structures of a RAG-like transposase during cut-and-paste transposition.
Liu C, Yang Y, Schatz DG
Nature 575 540-544 (2019)
PMID: 31723264
Related structures: EMD-20456

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109138 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Putative DNA-mediated transposase Chains: A, D
Molecule details ›
Chains: A, D
Length: 497 amino acids
Theoretical weight: 56.58 KDa
Source organism: Helicoverpa zea
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: B0F0C5 (Residues: 17-507; Coverage: 93%)
DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 16 nucleotides
Theoretical weight: 4.96 KDa
Source organism: Helicoverpa zea
Expression system: Not provided
DNA (5'-D(P*TP*TP*TP*TP*CP*GP*AP*TP*CP*CP*AP*CP*CP*GP*TP*G)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 16 nucleotides
Theoretical weight: 4.84 KDa
Source organism: Helicoverpa zea
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-20456
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

7 review citations

Structural insights into the evolution of the RAG recombinase.
Liu et al. (2022)
6 more

2 mentions without citation

Inner workings of RAG recombinase and its specialization for adaptive immunity.
Chen et al. (2021)
1 more