› 6prg

X-ray diffraction
2.35Å resolution

SBP RafE in complex with stachyose

Released:
DOI: 10.2210/pdb6prg/pdb
PDB entry 6prg coloured by chain, front view.
PDB entry 6prg coloured by chain, side view.
PDB entry 6prg coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Molecular analysis of an enigmatic Streptococcus pneumoniae virulence factor: The raffinose-family oligosaccharide utilization system.
Hobbs JK, Meier EPW, Pluvinage B, Mey MA, Boraston AB
J Biol Chem 294 17197-17208 (2019)
PMID: 31591266

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar ABC transporter, sugar-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 46.44 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2URJ7 (Residues: 24-419; Coverage: 95%)
Gene names: SP_1897, rafE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GLA, FRU
Carbohydrate polymer
2 bound ligands:
Ligand CIT 2 x CIT
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P212121
Unit cell:
a: 49.278Å b: 118.839Å c: 146.012Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.223 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

α-Galactosidase and Sucrose-Kinase Relationships in a Bi-functional AgaSK Enzyme Produced by the Human Gut Symbiont Ruminococcus gnavus E1.
Lafond et al. (2020)
3 more