› 6psm

X-ray diffraction
1.95Å resolution

Crystal structure of PsS1_19B C77S in complex with kappa-neocarrabiose

Released:
DOI: 10.2210/pdb6psm/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6psm coloured by chain, front view.
PDB entry 6psm coloured by chain, side view.
PDB entry 6psm coloured by chain, top view.
Source organism: Pseudoalteromonas fuliginea
Primary publication:
Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species.
Hettle AG, Hobbs JK, Pluvinage B, Vickers C, Abe KT, Salama-Alber O, McGuire BE, Hehemann JH, Hui JPM, Berrue F, Banskota A, Zhang J, Bottos EM, Van Hamme J, Boraston AB
OpenAccess logo Commun Biol 2 474 (2019)
PMID: 31886414

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
exo-4S-kappa carrageenan S1 sulfatase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 469 amino acids
Theoretical weight: 53.05 KDa
Source organism: Pseudoalteromonas fuliginea
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

6 bound ligands:
Ligand CA 6 x CA
Ligand G4S 6 x G4S
Ligand 9RN 6 x 9RN
Ligand CL 6 x CL
Ligand ZN 6 x ZN
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P31
Unit cell:
a: 93.05Å b: 93.05Å c: 300.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.
Bäumgen et al. (2021)
1 more

1 mention without citation

Sulfatases: Critical Enzymes for Algal Polysaccharide Processing.
Hettle et al. (2022)