› 6pt2

X-ray diffraction
2.8Å resolution

Crystal structure of the active delta opioid receptor in complex with the peptide agonist KGCHM07

Released:
DOI: 10.2210/pdb6pt2/pdb
PDB entry 6pt2 coloured by chain, front view.
PDB entry 6pt2 coloured by chain, side view.
PDB entry 6pt2 coloured by chain, top view.
Source organisms:
Primary publication:
Elucidating the active δ-opioid receptor crystal structure with peptide and small-molecule agonists.
Claff T, Yu J, Blais V, Patel N, Martin C, Wu L, Han GW, Holleran BJ, Van der Poorten O, White KL, Hanson MA, Sarret P, Gendron L, Cherezov V, Katritch V, Ballet S, Liu ZJ, Müller CE, Stevens RC
Sci Adv 5 eaax9115 (2019)
PMID: 31807708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154241 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Delta-type opioid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 51.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41143 (Residues: 41-338; Coverage: 80%)
Gene names: OPRD, OPRD1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Peptide agonist KGCHM07 Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 824 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CLR 1 x CLR
Ligand OLA 7 x OLA
Ligand OLC 1 x OLC
3 modified residues:
Ligand DI7 2 x DI7
Ligand DAR 2 x DAR
Ligand SAR 2 x SAR

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 48.96Å b: 140.88Å c: 158.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.282
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

15 review citations

Biased Opioid Ligands.
Faouzi et al. (2020)
14 more

21 mentions without citation

Allosteric Modulation of GPCRs of Class A by Cholesterol.
Jakubík et al. (2021)
20 more