› 6pu9

X-ray diffraction
1.7Å resolution

Crystal Structure of the Type B Chloramphenicol O-Acetyltransferase from Vibrio vulnificus

Released:
DOI: 10.2210/pdb6pu9/pdb
Model geometry
Fit model/data
PDB entry 6pu9 coloured by chain, front view.
PDB entry 6pu9 coloured by chain, side view.
PDB entry 6pu9 coloured by chain, top view.
Source organism: Vibrio vulnificus CMCP6
Primary publication:
Structural and functional characterization of three Type B and C chloramphenicol acetyltransferases from Vibrio species.
Alcala A, Ramirez G, Solis A, Kim Y, Tan K, Luna O, Nguyen K, Vazquez D, Ward M, Zhou M, Mulligan R, Maltseva N, Kuhn ML
Protein Sci 29 695-710 (2020)
PMID: 31762145

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloramphenicol acetyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 210 amino acids
Theoretical weight: 23.73 KDa
Source organism: Vibrio vulnificus CMCP6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3Q0KYH6 (Residues: 1-207; Coverage: 100%)
Gene name: VV2_0610
Sequence domains: Bacterial transferase hexapeptide (six repeats)

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P31
Unit cell:
a: 71.457Å b: 71.457Å c: 108.635Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Nanobiotics against antimicrobial resistance: harnessing the power of nanoscale materials and technologies.
Chakraborty et al. (2022)
1 more

1 mention without citation

Structural and functional characterization of three Type B and C chloramphenicol acetyltransferases from Vibrio species.
Alcala et al. (2020)