6pw2

X-ray diffraction
3.01Å resolution

Structural Basis for Cooperative Binding of EBNA1 to the Epstein-Barr Virus Dyad Symmetry Minimal Origin of Replication

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-120467 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Epstein-Barr nuclear antigen 1 Chains: A, B, C, D, I, J, K, L
Molecule details ›
Chains: A, B, C, D, I, J, K, L
Length: 147 amino acids
Theoretical weight: 16.01 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03211 (Residues: 461-607; Coverage: 23%)
Gene names: BKRF1, EBNA1
Sequence domains: Epstein Barr virus nuclear antigen-1, DNA-binding domain
DNA (62-MER) Chains: E, G
Molecule details ›
Chains: E, G
Length: 62 nucleotides
Theoretical weight: 19.13 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
DNA (62-MER) complementary DNA strand Chains: F, H
Molecule details ›
Chains: F, H
Length: 62 nucleotides
Theoretical weight: 19.08 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 64.118Å b: 283.384Å c: 63.899Å
α: 90° β: 89.8° γ: 90°
R-values:
R R work R free
0.22 0.22 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided