› 6pw3

X-ray diffraction
2.34Å resolution

LARP1 DM15 FYRE (F844Y, R847E) mutant bound to m7GpppG dinucleotide (capG)

Released:
DOI: 10.2210/pdb6pw3/pdb
PDB entry 6pw3 coloured by chain, front view.
PDB entry 6pw3 coloured by chain, side view.
PDB entry 6pw3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Capturing the Mechanism Underlying TOP mRNA Binding to LARP1.
Cassidy KC, Lahr RM, Kaminsky JC, Mack S, Fonseca BD, Das SR, Berman AJ, Durrant JD
Structure 27 1771-1781.e5 (2019)
PMID: 31676287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
La-related protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 162 amino acids
Theoretical weight: 19.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PKG0 (Residues: 873-1023; Coverage: 14%)
Gene names: KIAA0731, LARP, LARP1
Sequence domains: LARP1 HEAT repeat region

Ligands and Environments

2 bound ligands:
Ligand GTG 2 x GTG
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X17B1
Spacegroup: P21
Unit cell:
a: 58.582Å b: 87.355Å c: 72.89Å
α: 90° β: 93.367° γ: 90°
R-values:
R R work R free
0.224 0.22 0.267
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The plasticity of mRNA translation during cancer progression and therapy resistance.
Fabbri et al. (2021)
2 more

1 mention without citation

Capturing the Mechanism Underlying TOP mRNA Binding to LARP1.
Cassidy et al. (2019)