6pwf

Electron Microscopy
4.07Å resolution

Cryo-EM structure of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-120469 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H2A Chains: C, G
Complex ATPase-like protein Chain: K
Molecule details ›
Chain: K
Length: 640 amino acids
Theoretical weight: 73.46 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S9L5 (Residues: 77-134, 167-722; Coverage: 54%)
Gene name: CTHT_0046320
Sequence domains:
DNA (147-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.61 KDa
DNA (147-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.14 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.07Å
Relevant EMDB volumes: EMD-20507
Expression system: Escherichia coli