› 6px6

X-ray diffraction
3Å resolution

HLA-TCR complex

Released:
DOI: 10.2210/pdb6px6/pdb
PDB entry 6px6 coloured by chain, front view.
PDB entry 6px6 coloured by chain, side view.
PDB entry 6px6 coloured by chain, top view.
Source organisms:
Primary publication:
A molecular basis for the T cell response in HLA-DQ2.2 mediated celiac disease.
Ting YT, Dahal-Koirala S, Kim HSK, Qiao SW, Neumann RS, Lundin KEA, Petersen J, Reid HH, Sollid LM, Rossjohn J
Proc Natl Acad Sci U S A 117 3063-3073 (2020)
PMID: 31974305

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231541 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.96 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q08AS3 (Residues: 1-254; Coverage: 100%)
Gene name: HLA-DQA1
Sequence domains:
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 261 amino acids
Theoretical weight: 29.71 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: A0A0U5IHY9 (Residues: 1-261; Coverage: 100%)
Gene name: HLA-DQB1
Sequence domains:
Glutenin, low molecular weight subunit PTDUCD1 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: Triticum aestivum
Expression system: Not provided
UniProt:
  • Canonical: P16315 (Residues: 77-86; Coverage: 4%)
Ig-like domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 217 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: K7N5M3 (Residues: 3-31, 32-210; Coverage: 93%)
Gene names: B2M, HDCMA22P
Sequence domains:
T-cell receptor, T1005.2.56, beta chain Chain: E
Molecule details ›
Chain: E
Length: 256 amino acids
Theoretical weight: 28.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 72.534Å b: 99.416Å c: 72.565Å
α: 90° β: 118.264° γ: 90°
R-values:
R R work R free
0.208 0.205 0.257
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Not provided
  • Escherichia coli BL21

Quick links

Citations

11 review citations

Interplay Between Gluten, HLA, Innate and Adaptive Immunity Orchestrates the Development of Coeliac Disease.
Voisine et al. (2021)
10 more

7 mentions without citation

The Many Faces of CD4+ T Cells: Immunological and Structural Characteristics.
Chatzileontiadou et al. (2020)
6 more