› 6pxc

X-ray diffraction
1.6Å resolution

N-Terminal SH2 domain of the p120RasGAP bound to a p190RhoGAP phosphotyrosine peptide

Released:
DOI: 10.2210/pdb6pxc/pdb
PDB entry 6pxc coloured by chain, front view.
PDB entry 6pxc coloured by chain, side view.
PDB entry 6pxc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of p120RasGAP N-terminal SH2 domain in its apo form and in complex with a p190RhoGAP phosphotyrosine peptide.
Jaber Chehayeb R, Stiegler AL, Boggon TJ
PLoS One 14 e0226113 (2019)
PMID: 31891593

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149094 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras GTPase-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20936 (Residues: 174-280; Coverage: 10%)
Gene names: GAP, RASA, RASA1
Sequence domains: SH2 domain
Rho GTPase-activating protein 35 Chain: U
Molecule details ›
Chain: U
Length: 14 amino acids
Theoretical weight: 1.63 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NRY4 (Residues: 1100-1112; Coverage: 1%)
Gene names: ARHGAP35, GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 44.19Å b: 64.827Å c: 87.239Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Role of RASA1 in cancer: A review and update (Review).
Zhang et al. (2020)
2 more

5 mentions without citation

Crystal structures of p120RasGAP N-terminal SH2 domain in its apo form and in complex with a p190RhoGAP phosphotyrosine peptide.
Jaber Chehayeb et al. (2019)
4 more