› 6py8

X-ray diffraction
3.75Å resolution

Crystal structure of the RBPJ-NOTCH1-NRARP ternary complex bound to DNA

Released:
DOI: 10.2210/pdb6py8/pdb
PDB entry 6py8 coloured by chain, front view.
PDB entry 6py8 coloured by chain, side view.
PDB entry 6py8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Extension of the Notch intracellular domain ankyrin repeat stack by NRARP promotes feedback inhibition of Notch signaling.
Jarrett SM, Seegar TCM, Andrews M, Adelmant G, Marto JA, Aster JC, Blacklow SC
Sci Signal 12 (2019)
PMID: 31690634

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-120475 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Notch-regulated ankyrin repeat-containing protein Chains: B, G
Molecule details ›
Chains: B, G
Length: 114 amino acids
Theoretical weight: 12.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6K4 (Residues: 1-114; Coverage: 100%)
Gene name: NRARP
Sequence domains: Ankyrin repeats (3 copies)
Recombining binding protein suppressor of hairless Chains: C, E
Molecule details ›
Chains: C, E
Length: 444 amino acids
Theoretical weight: 50.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06330 (Residues: 23-466; Coverage: 89%)
Gene names: IGKJRB, IGKJRB1, RBPJ, RBPJK, RBPSUH
Sequence domains:
Notch 1 intracellular domain Chains: F, K
Molecule details ›
Chains: F, K
Length: 369 amino acids
Theoretical weight: 40.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1759-2127; Coverage: 15%)
Gene names: NOTCH1, TAN1
Sequence domains:
DNA Chains: A, X
Molecule details ›
Chains: A, X
Length: 16 nucleotides
Theoretical weight: 5 KDa
DNA Chains: D, Y
Molecule details ›
Chains: D, Y
Length: 16 nucleotides
Theoretical weight: 4.79 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 79.88Å b: 103.65Å c: 301.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.267 0.315
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Folding and Stability of Ankyrin Repeats Control Biological Protein Function.
Kumar et al. (2021)
1 more

6 mentions without citation

Biophysics of Notch Signaling.
Sprinzak et al. (2021)
5 more