› 6pzq

X-ray diffraction
2.7Å resolution

Structure of the human respiratory syncytial virus M2-1 protein in complex with a short positive-sense gene-end RNA

Released:
DOI: 10.2210/pdb6pzq/pdb
PDB entry 6pzq coloured by chain, front view.
PDB entry 6pzq coloured by chain, side view.
PDB entry 6pzq coloured by chain, top view.
Source organism: Human respiratory syncytial virus A2
Primary publication:
Structure of the Human Respiratory Syncytial Virus M2-1 Protein in Complex with a Short Positive-Sense Gene-End RNA.
Gao Y, Cao D, Pawnikar S, John KP, Ahn HM, Hill S, Ha JM, Parikh P, Ogilvie C, Swain A, Yang A, Bell A, Salazar A, Miao Y, Liang B
Structure 28 979-990.e4 (2020)
PMID: 32697936

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137907 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein M2-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 194 amino acids
Theoretical weight: 22.22 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Escherichia coli
UniProt:
  • Canonical: P04545 (Residues: 1-194; Coverage: 100%)
Gene name: M2-1
Sequence domains: Pneumovirus matrix protein 2 (M2)
RNA (5'-R(P*GP*UP*UP*AP*AP*U)-3') Chains: I, J
Molecule details ›
Chains: I, J
Length: 7 nucleotides
Theoretical weight: 2.21 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P41212
Unit cell:
a: 94.75Å b: 94.75Å c: 199.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The NEK family of serine/threonine kinases as a biomarker for cancer.
Panchal et al. (2023)
1 more

4 mentions without citation

Structural Insights into the Respiratory Syncytial Virus RNA Synthesis Complexes.
Cao et al. (2021)
3 more