› 6pzt

Electron Microscopy
3.46Å resolution

cryo-EM structure of human NKCC1

Released:
DOI: 10.2210/pdb6pzt/pdb
PDB entry 6pzt coloured by chain, front view.
PDB entry 6pzt coloured by chain, side view.
PDB entry 6pzt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human cation-chloride cotransporter NKCC1 determined by single-particle electron cryo-microscopy.
Yang X, Wang Q, Cao E
Nat Commun 11 1016 (2020)
PMID: 32081947
Related structures: EMD-20537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute carrier family 12 member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1212 amino acids
Theoretical weight: 131.67 KDa
Source organism: Homo sapiens
Expression system: Mammalian expression vector BsrGI-MCS-pcDNA3.1
UniProt:
  • Canonical: P55011 (Residues: 1-1212; Coverage: 100%)
Gene names: NKCC1, SLC12A2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-20537
Expression system: Mammalian expression vector BsrGI-MCS-pcDNA3.1

Quick links

Citations

9 review citations

Fluid transport in the brain.
Rasmussen et al. (2022)
8 more

7 mentions without citation

Cation-coupled chloride cotransporters: chemical insights and disease implications.
Portioli et al. (2021)
6 more