› 6q0k

Electron Microscopy
6.8Å resolution

Structure of a MAPK pathway complex

Released:
DOI: 10.2210/pdb6q0k/pdb
PDB entry 6q0k coloured by chain, front view.
PDB entry 6q0k coloured by chain, side view.
PDB entry 6q0k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Architecture of autoinhibited and active BRAF-MEK1-14-3-3 complexes.
Park E, Rawson S, Li K, Kim BW, Ficarro SB, Pino GG, Sharif H, Marto JA, Jeon H, Eck MJ
Nature 575 545-550 (2019)
PMID: 31581174
Related structures: EMD-20551

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147200 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 805 amino acids
Theoretical weight: 89.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P15056 (Residues: 1-766; Coverage: 100%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains:
14-3-3 protein zeta/delta Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 6.8Å
Relevant EMDB volumes: EMD-20551
Expression system: Homo sapiens

Quick links

Citations

32 review citations

RAF kinase dimerization: implications for drug discovery and clinical outcomes.
Brummer et al. (2020)
31 more

4 mentions without citation

Architecture of autoinhibited and active BRAF-MEK1-14-3-3 complexes.
Park et al. (2019)
3 more