› 6q3k

X-ray diffraction
1.5Å resolution

Engineered Human HLA_A2 MHC Class I molecule in complex with NV9 peptide

Released:
DOI: 10.2210/pdb6q3k/pdb
PDB entry 6q3k coloured by chain, front view.
PDB entry 6q3k coloured by chain, side view.
PDB entry 6q3k coloured by chain, top view.
Source organisms:
Primary publication:
Engineered Human HLA_A2MHC Class I molecule in complex with NV9 peptide
Meijers R, Anjanappa R, Springer S, Garcia-Alai M
Sci Immunol (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137800 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 24-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
ASN-LEU-VAL-PRO-MET-VAL-ALA-THR-VAL Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 943 Da
Source organism: Cytomegalovirus
Expression system: synthetic construct

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand CO 7 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 60.158Å b: 80.575Å c: 109.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.2
Expression systems:
  • Escherichia coli
  • synthetic construct

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