› 6q58

X-ray diffraction
1.5Å resolution

Copper loading to a cytosolic copper storage protein from Streptomyces lividans (five coppers)

Released:
DOI: 10.2210/pdb6q58/pdb
PDB entry 6q58 coloured by chain, front view.
PDB entry 6q58 coloured by chain, side view.
PDB entry 6q58 coloured by chain, top view.
Source organism: Streptomyces lividans 1326
Primary publication:
A Histidine Residue and a Tetranuclear Cuprous-thiolate Cluster Dominate the Copper Loading Landscape of a Copper Storage Protein from Streptomyces lividans.
Straw ML, Hough MA, Wilson MT, Worrall JAR
Chemistry 25 10678-10688 (2019)
PMID: 31111982

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Four-helix bundle copper-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 12.54 KDa
Source organism: Streptomyces lividans 1326
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X8F4 (Residues: 17-136; Coverage: 88%)
Gene names: SCE39.31c, SCO3281
Sequence domains: Domain of Unknown Function (DUF326)

Ligands and Environments

1 bound ligand:
Ligand CU1 15 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 93.051Å b: 93.051Å c: 212.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.214
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Mercaptothiacalixarenes Steer 24 Copper(I) Centers to form a Hollow-Sphere Structure Featuring Cu2 S2 Motifs with Exceptionally Short Cu⋅⋅⋅Cu Distances.
Frank et al. (2020)
1 more