› 6q5a

X-ray diffraction
1.55Å resolution

Crystal structure of Cryptosporidium hominis CPSF3 in the apo form

Released:
DOI: 10.2210/pdb6q5a/pdb
PDB entry 6q5a coloured by chain, front view.
PDB entry 6q5a coloured by chain, side view.
PDB entry 6q5a coloured by chain, top view.
Source organism: Cryptosporidium hominis
Primary publication:
Metal-captured inhibition of pre-mRNA processing activity by CPSF3 controls Cryptosporidium infection.
Swale C, Bougdour A, Gnahoui-David A, Tottey J, Georgeault S, Laurent F, Palencia A, Hakimi MA
Sci Transl Med 11 (2019)
PMID: 31694928

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-Casp domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 54.01 KDa
Source organism: Cryptosporidium hominis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S4TJL4 (Residues: 1-482; Coverage: 62%)
Gene name: CHUDEA8_460
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
Ligand IPA 3 x IPA
Ligand MG 1 x MG
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P6522
Unit cell:
a: 90.36Å b: 90.36Å c: 238.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.138 0.179
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Recent molecular insights into canonical pre-mRNA 3'-end processing.
Sun et al. (2020)
7 more