› 6q8i

X-ray diffraction
3.17Å resolution

Nterminal domain of human SMU1 in complex with human REDmid

Released:
DOI: 10.2210/pdb6q8i/pdb
PDB entry 6q8i coloured by chain, front view.
PDB entry 6q8i coloured by chain, side view.
PDB entry 6q8i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Destabilization of the human RED-SMU1 splicing complex as a basis for host-directed antiinfluenza strategy.
Ashraf U, Tengo L, Le Corre L, Fournier G, Busca P, McCarthy AA, Rameix-Welti MA, Gravier-Pelletier C, Ruigrok RWH, Jacob Y, Vidalain PO, Pietrancosta N, Crépin T, Naffakh N
Proc Natl Acad Sci U S A 116 10968-10977 (2019)
PMID: 31076555

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171543 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD40 repeat-containing protein SMU1 Chains: A, B, E, F, I, J, M, N
Molecule details ›
Chains: A, B, E, F, I, J, M, N
Length: 514 amino acids
Theoretical weight: 57.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TAY7 (Residues: 2-513; Coverage: 100%)
Gene name: SMU1
Sequence domains:
Protein Red Chains: C, D, G, H, L, O, P
Molecule details ›
Chains: C, D, G, H, L, O, P
Length: 557 amino acids
Theoretical weight: 65.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13123 (Residues: 1-557; Coverage: 100%)
Gene names: IK, RED, RER
Sequence domains:
Protein Red Chain: K
Molecule details ›
Chain: K
Length: 557 amino acids
Theoretical weight: 65.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13123 (Residues: 1-557; Coverage: 100%)
Gene names: IK, RED, RER
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 127.983Å b: 68.163Å c: 145.296Å
α: 90° β: 109.4° γ: 90°
R-values:
R R work R free
0.257 0.256 0.277
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Benchmarking the Accuracy of AlphaFold 2 in Loop Structure Prediction.
Stevens et al. (2022)
2 more

1 mention without citation

Destabilization of the human RED-SMU1 splicing complex as a basis for host-directed antiinfluenza strategy.
Ashraf et al. (2019)