› 6q9r

X-ray diffraction
2.73Å resolution

Crystal structure of the pathological N184K variant of calcium-free human gelsolin

Released:
DOI: 10.2210/pdb6q9r/pdb
Model geometry
Fit model/data
PDB entry 6q9r coloured by chain, front view.
PDB entry 6q9r coloured by chain, side view.
PDB entry 6q9r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties.
de Rosa M, Barbiroli A, Bonì F, Scalone E, Mattioni D, Vanoni MA, Patrone M, Bollati M, Mastrangelo E, Giorgino T, Milani M
Eur Biophys J 49 11-19 (2020)
PMID: 31724080

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gelsolin Chains: A, B
Molecule details ›
Chains: A, B
Length: 778 amino acids
Theoretical weight: 85.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 28-782; Coverage: 100%)
  • Best match: P06396-3 (Residues: 9-742)
Gene name: GSN
Sequence domains: Gelsolin repeat

Ligands and Environments

4 bound ligands:
Ligand CL 9 x CL
Ligand GOL 17 x GOL
Ligand SO4 2 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P4212
Unit cell:
a: 169.848Å b: 169.848Å c: 150.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.26
Expression system: Escherichia coli

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Citations

2 citation in other articles

A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation.
Bollati et al. (2021)
1 more