› 6qas

X-ray diffraction
1.75Å resolution

Crystal structure of ULK1 in complexed with PF-03814735

Released:
DOI: 10.2210/pdb6qas/pdb
PDB entry 6qas coloured by chain, front view.
PDB entry 6qas coloured by chain, side view.
PDB entry 6qas coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conservation of structure, function and inhibitor binding in UNC-51-like kinase 1 and 2 (ULK1/2).
Chaikuad A, Koschade SE, Stolz A, Zivkovic K, Pohl C, Shaid S, Ren H, Lambert LJ, Cosford NDP, Brandts CH, Knapp S
Biochem J 476 875-887 (2019)
PMID: 30782972

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase ULK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75385 (Residues: 1-283; Coverage: 27%)
Gene names: KIAA0722, ULK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 6 x SO4
Ligand CIT 2 x CIT
Ligand GOL 11 x GOL
Ligand EDO 12 x EDO
Ligand 34W 3 x 34W
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43212
Unit cell:
a: 74.241Å b: 74.241Å c: 222.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.197
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Targeting Autophagy in Cancer: Recent Advances and Future Directions.
Amaravadi et al. (2019)
7 more

5 mentions without citation

Mitochondrial quality control in the brain: The physiological and pathological roles.
Shen et al. (2022)
4 more