› 6qcm

Electron Microscopy
4.21Å resolution

Cryo em structure of the Listeria stressosome

Released:
DOI: 10.2210/pdb6qcm/pdb
PDB entry 6qcm coloured by chain, front view.
PDB entry 6qcm coloured by chain, side view.
PDB entry 6qcm coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
The cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation.
Williams AH, Redzej A, Rolhion N, Costa TRD, Rifflet A, Waksman G, Cossart P
Nat Commun 10 3005 (2019)
PMID: 31285450
Related structures: EMD-4508

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187003 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
STAS domain-containing protein Chains: AB, BC, DC, EB, FA, FB, FC, GC, HC, IC, K, KB, L, LB, M, OB, P, PB, Q, SB, T, TB, U, UB, V, VB, W, X, Y, Z
Molecule details ›
Chains: AB, BC, DC, EB, FA, FB, FC, GC, HC, IC, K, KB, L, LB, M, OB, P, PB, Q, SB, T, TB, U, UB, V, VB, W, X, Y, Z
Length: 128 amino acids
Theoretical weight: 13.94 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y8K9 (Residues: 148-275; Coverage: 46%)
Gene name: RsbR
Sequence domains: STAS domain
STAS domain-containing protein Chains: CB, DB, E, F, GB, IB
Molecule details ›
Chains: CB, DB, E, F, GB, IB
Length: 129 amino acids
Theoretical weight: 14.07 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y8K9 (Residues: 147-275; Coverage: 46%)
Gene name: RsbR
Sequence domains: STAS domain
STAS domain-containing protein Chains: A, B, C, D, I, J, N, O, R, S, a, b, c, d, e, f, g, h, i, j
Molecule details ›
Chains: A, B, C, D, I, J, N, O, R, S, a, b, c, d, e, f, g, h, i, j
Length: 118 amino acids
Theoretical weight: 12.61 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92DC5 (Residues: 1-118; Coverage: 100%)
Gene name: rsbS
Sequence domains: STAS domain
STAS domain-containing protein Chain: AD
Molecule details ›
Chain: AD
Length: 123 amino acids
Theoretical weight: 13.48 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y8K9 (Residues: 140-275; Coverage: 44%)
Gene name: RsbR
Sequence domains: STAS domain
STAS domain-containing protein Chains: CD, FD, HD
Molecule details ›
Chains: CD, FD, HD
Length: 136 amino acids
Theoretical weight: 14.86 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8Y8K9 (Residues: 140-275; Coverage: 49%)
Gene name: RsbR
Sequence domains: STAS domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.21Å
Relevant EMDB volumes: EMD-4508
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Towards the Idea of Molecular Brains.
Timsit et al. (2021)
3 more

3 mentions without citation

Bacterial Small Membrane Proteins: the Swiss Army Knife of Regulators at the Lipid Bilayer.
Yadavalli et al. (2022)
2 more