› 6qee

Electron Microscopy
3.9Å resolution

Nanodisc reconstituted Human-mouse chimeric ABCB1 (ABCB1HM)-EQ mutant in complex with UIC2 Fab and Zosuquidar.

Released:
DOI: 10.2210/pdb6qee/pdb
PDB entry 6qee coloured by chain, front view.
PDB entry 6qee coloured by chain, side view.
PDB entry 6qee coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into substrate and inhibitor discrimination by human P-glycoprotein.
Alam A, Kowal J, Broude E, Roninson I, Locher KP
Science 363 753-756 (2019)
PMID: 30765569
Related structures: EMD-4536

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211761 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ABCB1HM-EQ Chain: A
Molecule details ›
Chain: A
Length: 1300 amino acids
Theoretical weight: 143.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UIC2 Antigen Binding Fragment Light chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 24.32 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
UIC2 Antigen Binding Fragment Heavy Chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 24.38 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

4 bound ligands:
Ligand NAG 3 x NAG
Ligand ZQU 2 x ZQU
Ligand CLR 7 x CLR
Ligand 3PE 1 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-4536
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

28 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
27 more

23 mentions without citation

Mechanics and pharmacology of substrate selection and transport by eukaryotic ABC exporters.
Srikant et al. (2019)
22 more