› 6qh3

X-ray diffraction
2.9Å resolution

Catalytic domain of the human ubiquitin-conjugating enzyme UBE2S C118M

Released:
DOI: 10.2210/pdb6qh3/pdb
PDB entry 6qh3 coloured by chain, front view.
PDB entry 6qh3 coloured by chain, side view.
PDB entry 6qh3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Liess AKL, Kucerova A, Schweimer K, Yu L, Roumeliotis TI, Diebold M, Dybkov O, Sotriffer C, Urlaub H, Choudhary JS, Mansfeld J, Lorenz S
Structure 27 1195-1210.e7 (2019)
PMID: 31230944

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 S Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16763 (Residues: 1-156; Coverage: 70%)
Gene names: E2EPF, OK/SW-cl.73, UBE2S
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P65
Unit cell:
a: 84.619Å b: 84.619Å c: 87.832Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Role of Conformational Dynamics in the Recognition and Regulation of Ubiquitination.
Khago et al. (2020)
2 more

1 mention without citation

Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S.
Liess et al. (2020)