› 6qi9

Electron Microscopy
4.63Å resolution

Truncated human R2TP complex, structure 4 (ADP-empty)

Released:
DOI: 10.2210/pdb6qi9/pdb
PDB entry 6qi9 coloured by chain, front view.
PDB entry 6qi9 coloured by chain, side view.
PDB entry 6qi9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM.
Muñoz-Hernández H, Pal M, Rodríguez CF, Fernandez-Leiro R, Prodromou C, Pearl LH, Llorca O
Sci Adv 5 eaaw1616 (2019)
PMID: 31049401
Related structures: EMD-4553

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195084 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 456 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
RuvB-like 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 463 amino acids
Theoretical weight: 51.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.63Å
Relevant EMDB volumes: EMD-4553
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The functions and regulation of heat shock proteins; key orchestrators of proteostasis and the heat shock response.
Lang et al. (2021)
4 more

1 mention without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)