› 6qmp

X-ray diffraction
2Å resolution

NF-YB/C Heterodimer in Complex with NF-YA Peptide

Released:
DOI: 10.2210/pdb6qmp/pdb
PDB entry 6qmp coloured by chain, front view.
PDB entry 6qmp coloured by chain, side view.
PDB entry 6qmp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Constrained Peptides with Fine-Tuned Flexibility Inhibit NF-Y Transcription Factor Assembly.
Jeganathan S, Wendt M, Kiehstaller S, Brancaccio D, Kuepper A, Pospiech N, Carotenuto A, Novellino E, Hennig S, Grossmann TN
Angew Chem Int Ed Engl 58 17351-17358 (2019)
PMID: 31539186

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149991 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear transcription factor Y subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 31 amino acids
Theoretical weight: 3.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P23511 (Residues: 267-295; Coverage: 8%)
Gene name: NFYA
Sequence domains: CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Nuclear transcription factor Y subunit beta Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25208 (Residues: 51-143; Coverage: 45%)
Gene names: HAP3, NFYB
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Nuclear transcription factor Y subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 11.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13952 (Residues: 27-120; Coverage: 21%)
Gene name: NFYC
Sequence domains: Core histone H2A/H2B/H3/H4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 52.9Å b: 52.9Å c: 134.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.241
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

1 review citation

Peptide-based inhibitors of protein-protein interactions: biophysical, structural and cellular consequences of introducing a constraint.
Wang et al. (2021)
13 other articles

1 mention without citation

Constrained Peptides with Fine-Tuned Flexibility Inhibit NF-Y Transcription Factor Assembly.
Jeganathan et al. (2019)