› 6qnn

X-ray diffraction
2.03Å resolution

CLATHRIN HEAVY CHAIN N-TERMINAL DOMAIN BOUND TO GTSE1 LIDL MOTIF

Released:
DOI: 10.2210/pdb6qnn/pdb
PDB entry 6qnn coloured by chain, front view.
PDB entry 6qnn coloured by chain, side view.
PDB entry 6qnn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Clathrin's adaptor interaction sites are repurposed to stabilize microtubules during mitosis.
Rondelet A, Lin YC, Singh D, Porfetye AT, Thakur HC, Hecker A, Brinkert P, Schmidt N, Bendre S, Müller F, Mazul L, Widlund PO, Bange T, Hiller M, Vetter IR, Bird AW
J Cell Biol 219 (2020)
PMID: 31932847

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169457 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Clathrin heavy chain 1 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 40.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00610 (Residues: 1-364; Coverage: 22%)
Gene names: CLH17, CLTC, CLTCL2, KIAA0034
Sequence domains:
G2 and S phase-expressed protein 1 Chain: B
Molecule details ›
Chain: B
Length: 66 amino acids
Theoretical weight: 7.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NYZ3 (Residues: 661-726; Coverage: 9%)
Gene name: GTSE1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43
Unit cell:
a: 51.987Å b: 51.987Å c: 154.094Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs.
Mihalič et al. (2023)
3 more

1 mention without citation

Clathrin's adaptor interaction sites are repurposed to stabilize microtubules during mitosis.
Rondelet et al. (2020)