› 6qoy

X-ray diffraction
1.9Å resolution

Crystal structure of L1 protease Lysobacter sp. XL1 in complex with AEBSF

Released:
DOI: 10.2210/pdb6qoy/pdb
PDB entry 6qoy coloured by chain, front view.
PDB entry 6qoy coloured by chain, side view.
PDB entry 6qoy coloured by chain, top view.
Source organism: Lysobacter sp. XL1
Primary publication:
Serine bacteriolytic protease L1 of Lysobacter sp. XL1 complexed with protease inhibitor AEBSF: features of interaction
Kudryakova I, Gabdulkhakov A, Tishchenko S, Afoshin A, Vasilyeva N
Process Biochem (2019)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lytic endopeptidase preproenzyme Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 19.85 KDa
Source organism: Lysobacter sp. XL1
Expression system: Escherichia coli
UniProt:
  • Canonical: D2K8B3 (Residues: 200-398; Coverage: 55%)
Gene name: alpA
Sequence domains: Trypsin

Ligands and Environments

7 bound ligands:
Ligand SO4 8 x SO4
Ligand AES 4 x AES
Ligand GOL 5 x GOL
Ligand PGE 1 x PGE
Ligand JAT 3 x JAT
Ligand CL 2 x CL
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 41.855Å b: 122.553Å c: 78.988Å
α: 90° β: 98.64° γ: 90°
R-values:
R R work R free
0.202 0.2 0.24
Expression system: Escherichia coli

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