› 6qpr

X-ray diffraction
1.45Å resolution

Rhizomucor miehei lipase propeptide complex, Ser95/Ile96 deletion mutant

Released:
DOI: 10.2210/pdb6qpr/pdb
PDB entry 6qpr coloured by chain, front view.
PDB entry 6qpr coloured by chain, side view.
PDB entry 6qpr coloured by chain, top view.
Source organism: Rhizomucor miehei
Primary publication:
Novel Inhibitory Function of the Rhizomucor miehei Lipase Propeptide and Three-Dimensional Structures of Its Complexes with the Enzyme.
Moroz OV, Blagova E, Reiser V, Saikia R, Dalal S, Jørgensen CI, Bhatia VK, Baunsgaard L, Andersen B, Svendsen A, Wilson KS
ACS Omega 4 9964-9975 (2019)
PMID: 31460089

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 39.43 KDa
Source organism: Rhizomucor miehei
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P19515 (Residues: 1-363; Coverage: 99%)
Sequence domains: Lipase (class 3)

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 98.696Å b: 61.374Å c: 62.814Å
α: 90° β: 111.85° γ: 90°
R-values:
R R work R free
0.128 0.126 0.165
Expression system: Aspergillus oryzae

Quick links

Citations

1 review citation

Cold Active Lipases: Biocatalytic Tools for Greener Technology.
Mhetras et al. (2021)
2 other articles

4 mentions without citation

Novel Inhibitory Function of the Rhizomucor miehei Lipase Propeptide and Three-Dimensional Structures of Its Complexes with the Enzyme.
Moroz et al. (2019)
3 more