› 6qrj

X-ray diffraction
2.65Å resolution

Crystal structure of ShkA full-length in complex with AMPPNP

Released:
DOI: 10.2210/pdb6qrj/pdb
PDB entry 6qrj coloured by chain, front view.
PDB entry 6qrj coloured by chain, side view.
PDB entry 6qrj coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Primary publication:
Hybrid histidine kinase activation by cyclic di-GMP-mediated domain liberation.
Dubey BN, Agustoni E, Böhm R, Kaczmarczyk A, Mangia F, von Arx C, Jenal U, Hiller S, Plaza-Menacho I, Schirmer T
Proc Natl Acad Sci U S A 117 1000-1008 (2020)
PMID: 31882446

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 50.3 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ABT2 (Residues: 13-496; Coverage: 94%)
Gene names: CC0138, CC_0138, shkA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P4322
Unit cell:
a: 64.504Å b: 64.504Å c: 260.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.275
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The World of Cyclic Dinucleotides in Bacterial Behavior.
Aline Dias da et al. (2020)
1 more

4 mentions without citation

Diversity in Sensing and Signaling of Bacterial Sensor Histidine Kinases.
Ishii et al. (2021)
3 more