› 6qus

Electron Microscopy
3.7Å resolution

HsCKK (human CAMSAP1) decorated 13pf taxol-GDP microtubule

Released:
DOI: 10.2210/pdb6qus/pdb
PDB entry 6qus coloured by chain, front view.
PDB entry 6qus coloured by chain, side view.
PDB entry 6qus coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of microtubule minus end preference in CAMSAP CKK domains.
Atherton J, Luo Y, Xiang S, Yang C, Rai A, Jiang K, Stangier M, Vemu A, Cook AD, Wang S, Roll-Mecak A, Steinmetz MO, Akhmanova A, Baldus M, Moores CA
Nat Commun 10 5236 (2019)
PMID: 31748546
Related structures: EMD-4643

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-139591 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: O, X
Molecule details ›
Chains: O, X
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68363 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Calmodulin-regulated spectrin-associated protein 1 Chain: I
Molecule details ›
Chain: I
Length: 174 amino acids
Theoretical weight: 19.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T5Y3 (Residues: 1462-1602; Coverage: 9%)
Gene name: CAMSAP1
Sequence domains: Microtubule-binding calmodulin-regulated spectrin-associated
Tubulin beta chain Chains: S, U
Molecule details ›
Chains: S, U
Length: 444 amino acids
Theoretical weight: 49.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07437 (Residues: 1-444; Coverage: 100%)
Gene names: OK/SW-cl.56, TUBB, TUBB5
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand GDP 2 x GDP
Ligand TA1 2 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4643
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Microtubule Organization Is Essential for Maintaining Cellular Morphology and Function.
Huang et al. (2022)
2 more

1 mention without citation

A microtubule RELION-based pipeline for cryo-EM image processing.
Cook et al. (2020)