› 6qwh

X-ray diffraction
2.9Å resolution

The Transcriptional Regulator PrfA-L140H mutant from Listeria Monocytogenes in complex with a 30-bp operator PrfA-box motif

Released:
DOI: 10.2210/pdb6qwh/pdb
PDB entry 6qwh coloured by chain, front view.
PDB entry 6qwh coloured by chain, side view.
PDB entry 6qwh coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
Hansen S, Hall M, Grundström C, Brännström K, Sauer-Eriksson AE, Johansson J
J Bacteriol 202 (2020)
PMID: 32179627

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120507 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.48 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
DNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 9.26 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided
DNA (30-MER) Chain: D
Molecule details ›
Chain: D
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P43212
Unit cell:
a: 78.273Å b: 78.273Å c: 265.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.254 0.292
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

NrnA Is a Linear Dinucleotide Phosphodiesterase with Limited Function in Cyclic Dinucleotide Metabolism in Listeria monocytogenes.
Gall et al. (2022)

1 mention without citation

A Novel Growth-Based Selection Strategy Identifies New Constitutively Active Variants of the Major Virulence Regulator PrfA in Listeria monocytogenes.
Hansen et al. (2020)