› 6qxz

Solution NMR

Solution structure of the ASHH2 CW domain with the N-terminal histone H3 tail mimicking peptide monomethylated on lysine 4

Released:
DOI: 10.2210/pdb6qxz/pdb
PDB entry 6qxz coloured by chain, ensemble of 20 models, front view.
PDB entry 6qxz coloured by chain, ensemble of 20 models, side view.
PDB entry 6qxz coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection.
Dobrovolska O, Brilkov M, Madeleine N, Ødegård-Fougner Ø, Strømland Ø, Martin SR, De Marco V, Christodoulou E, Teigen K, Isaksson J, Underhaug J, Reuter N, Aalen RB, Aasland R, Halskau Ø
FEBS J 287 4458-4480 (2020)
PMID: 32083791

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159409 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase ASHH2 Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 8.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2LAE1 (Residues: 861-928; Coverage: 4%)
Gene names: ASHH2, At1g77300, EFS, LAZ2, SDG8, SET8, T14N5.15
Sequence domains: CW-type Zinc Finger
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-9; Coverage: 6%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand MLZ 1 x MLZ

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: distance geometry
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Methyltransferases: Functions and Applications.
Abdelraheem et al. (2022)
1 more

2 mentions without citation

The Arabidopsis (ASHH2) CW domain binds monomethylated K4 of the histone H3 tail through conformational selection.
Dobrovolska et al. (2020)
1 more