› 6qz1

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed: 
Ethylene terephthalate + H(2)O = terephthalate + ethylene glycol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mono(2-hydroxyethyl) terephthalate hydrolase Chain: A
Molecule details ›
Chain: A
Length: 564 amino acids
Theoretical weight: 59.24 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P8E7 (Residues: 40-603; Coverage: 96%)
Gene name: ISF6_0224
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

2 bound ligands:
Ligand BEZ 1 x BEZ
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 77.195Å b: 89.875Å c: 91.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.192
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Mechanism-Based Design of Efficient PET Hydrolases.
Wei et al. (2022)
25 more

3 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
2 more