› 6qz4

X-ray diffraction
1.8Å resolution

Function and Biology Details

Reaction catalysed: 
Ethylene terephthalate + H(2)O = terephthalate + ethylene glycol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mono(2-hydroxyethyl) terephthalate hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 611 amino acids
Theoretical weight: 64.22 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P8E7 (Residues: 1-603; Coverage: 100%)
Gene name: ISF6_0224
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 90.212Å b: 92.804Å c: 159.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.205
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Mechanism-Based Design of Efficient PET Hydrolases.
Wei et al. (2022)
26 more

4 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
3 more