6qzf

Electron Microscopy
3.8Å resolution

The cryo-EM structure of the collar complex and tail axis in genome emptied bacteriophage phi29

Released:
Source organism: Salasvirus phi29
Primary publication:
Structural assembly of the tailed bacteriophage ϕ29.
Nat Commun 10 2366 (2019)
PMID: 31147544
Related structures: EMD-4685

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
PDBe Complex ID:
PDB-CPX-137598 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Portal protein Chains: 0a, 0b, 0c, 0d, 0e, 0f, 0g, 0h, 0i, 0j, 0k, 0l
Molecule details ›
Chains: 0a, 0b, 0c, 0d, 0e, 0f, 0g, 0h, 0i, 0j, 0k, 0l
Length: 309 amino acids
Theoretical weight: 35.92 KDa
Source organism: Salasvirus phi29
UniProt:
  • Canonical: P04332 (Residues: 1-309; Coverage: 100%)
Gene name: 10
Sequence domains: Phage Connector (GP10)
Proximal tail tube connector protein Chains: 0A, 0B, 0C, 0D, 0E, 0F, 0G, 0H, 0I, 0J, 0K, 0L
Molecule details ›
Chains: 0A, 0B, 0C, 0D, 0E, 0F, 0G, 0H, 0I, 0J, 0K, 0L
Length: 293 amino acids
Theoretical weight: 33.84 KDa
Source organism: Salasvirus phi29
UniProt:
  • Canonical: P68930 (Residues: 1-293; Coverage: 100%)
Gene name: 11
Pre-neck appendage protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j
Length: 854 amino acids
Theoretical weight: 92.19 KDa
Source organism: Salasvirus phi29
UniProt:
  • Canonical: P20345 (Residues: 1-854; Coverage: 100%)
Gene name: 12
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-4685