› 6qzh

X-ray diffraction
2.1Å resolution

Structure of the human CC Chemokine Receptor 7 in complex with the intracellular allosteric antagonist Cmp2105 and the insertion protein Sialidase NanA

Released:
DOI: 10.2210/pdb6qzh/pdb
PDB entry 6qzh coloured by chain, front view.
PDB entry 6qzh coloured by chain, side view.
PDB entry 6qzh coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Allosteric Ligand Recognition in the Human CC Chemokine Receptor 7.
Jaeger K, Bruenle S, Weinert T, Guba W, Muehle J, Miyazaki T, Weber M, Furrer A, Haenggi N, Tetaz T, Huang CY, Mattle D, Vonach JM, Gast A, Kuglstatter A, Rudolph MG, Nogly P, Benz J, Dawson RJP, Standfuss J
Cell 178 1222-1230.e10 (2019)
PMID: 31442409

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-C chemokine receptor type 7; Sialidase A Chain: A
Molecule details ›
Chain: A
Length: 785 amino acids
Theoretical weight: 88.34 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32248 (Residues: 46-247; Coverage: 57%)
  • Canonical: P62576 (Residues: 322-791, 796-802; Coverage: 49%)
Gene names: CCR7, CMKBR7, EBI1, EVI1, nanA, spr1536
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand PGE 1 x PGE
Ligand JLW 1 x JLW
Ligand NA 1 x NA
Ligand TAR 2 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 79.124Å b: 128.809Å c: 100.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Ligands of Adrenergic Receptors: A Structural Point of View.
Wu et al. (2021)
9 more

11 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
10 more