› 6r35

X-ray diffraction
1.8Å resolution

Structure of the LecB lectin from Pseudomonas aeruginosa strain PAO1 in complex with lewis x tetrasaccharide

Released:
DOI: 10.2210/pdb6r35/pdb
PDB entry 6r35 coloured by chain, front view.
PDB entry 6r35 coloured by chain, side view.
PDB entry 6r35 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Induction of rare conformation of oligosaccharide by binding to calcium-dependent bacterial lectin: X-ray crystallography and modelling study.
Lepsik M, Sommer R, Kuhaudomlarp S, Lelimousin M, Paci E, Varrot A, Titz A, Imberty A
Eur J Med Chem 177 212-220 (2019)
PMID: 31146126

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 114 amino acids
Theoretical weight: 11.73 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 2-115; Coverage: 99%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand CA 8 x CA
Ligand SO4 2 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 52.603Å b: 72.481Å c: 62.061Å
α: 90° β: 114.62° γ: 90°
R-values:
R R work R free
0.134 0.132 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Increasing the Affinity of an O-Antigen Polysaccharide Binding Site in Shigella flexneri Bacteriophage Sf6 Tailspike Protein.
Kunstmann et al. (2020)
1 more