› 6raw

Electron Microscopy
3.7Å resolution

D. melanogaster CMG-DNA, State 1A

Released:
DOI: 10.2210/pdb6raw/pdb
PDB entry 6raw coloured by chain, front view.
PDB entry 6raw coloured by chain, side view.
PDB entry 6raw coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep 28 2673-2688.e8 (2019)
PMID: 31484077
Related structures: EMD-4785

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120536 (preferred)
Entry contents:
11 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (13 distinct):
DNA replication licensing factor Mcm2 Chain: 2
Molecule details ›
Chain: 2
Length: 887 amino acids
Theoretical weight: 100.54 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49735 (Residues: 1-887; Coverage: 100%)
Gene names: CG7538, Mcm2
Sequence domains:
DNA replication licensing factor Mcm3 Chain: 3
Molecule details ›
Chain: 3
Length: 819 amino acids
Theoretical weight: 91.05 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9XYU1 (Residues: 1-819; Coverage: 100%)
Gene names: CG4206, Mcm3, Mcm3-RA
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 866 amino acids
Theoretical weight: 96.74 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q26454 (Residues: 1-866; Coverage: 100%)
Gene names: CG1616, dpa
Sequence domains:
DNA replication licensing factor Mcm5 Chain: 5
Molecule details ›
Chain: 5
Length: 733 amino acids
Theoretical weight: 82.38 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VGW6 (Residues: 1-733; Coverage: 100%)
Gene names: CG4082, Mcm5
Sequence domains:
DNA replication licensing factor Mcm6 Chain: 6
Molecule details ›
Chain: 6
Length: 817 amino acids
Theoretical weight: 92.47 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9V461 (Residues: 1-817; Coverage: 100%)
Gene names: CG4039, Mcm6
Sequence domains:
DNA replication licensing factor Mcm7 Chain: 7
Molecule details ›
Chain: 7
Length: 720 amino acids
Theoretical weight: 81.4 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9XYU0 (Residues: 1-720; Coverage: 100%)
Gene names: CG4978, Mcm7
Sequence domains:
LD35753p Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 65.97 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O96989 (Residues: 1-575; Coverage: 100%)
Gene names: CDC45, CDC45L, CG3658, Cdc45, D, DmCdc45, Dmel\CG3658, Dmel_CG3658, EG:BACR7A4.11, anon-1Ec, cdc45, dCDC45, dCDC45L
Sequence domains: CDC45-like protein
DNA replication complex GINS protein PSF1 Chain: H
Molecule details ›
Chain: H
Length: 202 amino acids
Theoretical weight: 23.33 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W0I7 (Residues: 1-202; Coverage: 100%)
Gene names: CG9187, CG9187-PA, Dmel\CG9187, Dmel_CG9187, PSF1, Psf1, psf1
Sequence domains: GINS complex protein
Probable DNA replication complex GINS protein PSF2 Chain: L
Molecule details ›
Chain: L
Length: 203 amino acids
Theoretical weight: 23.14 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VQY9 (Residues: 1-203; Coverage: 100%)
Gene names: CG18013, Psf2
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF3 Chain: M
Molecule details ›
Chain: M
Length: 212 amino acids
Theoretical weight: 24.83 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9W2V7 (Residues: 1-212; Coverage: 100%)
Gene names: CG2222, Dmel\CG2222, Dmel_CG2222, PSF3, Psf3
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: N
Molecule details ›
Chain: N
Length: 228 amino acids
Theoretical weight: 26.15 KDa
Source organism: Drosophila melanogaster
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9VBI1 (Residues: 1-228; Coverage: 100%)
Gene names: CG14549, Dmel\CG14549, Dmel_CG14549, SLD5, Sld5, anon-WO0172774.61
Sequence domains:
DNA Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.93 KDa
DNA (5'-D(P*TP*CP*GP*AP*TP*CP*GP*AP*TP*CP*GP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 13 nucleotides
Theoretical weight: 3.97 KDa

Ligands and Environments

2 bound ligands:
Ligand ATP 3 x ATP
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4785
Expression system: Trichoplusia ni

Quick links

Citations

14 review citations

Mechanisms of hexameric helicases.
Fernandez et al. (2021)
13 more

2 mentions without citation

DNA replication protein Cdc45 directly interacts with PCNA via its PIP box in Leishmania donovani and the Cdc45 PIP box is essential for cell survival.
Yadav et al. (2020)
1 more