6rj9

Electron Microscopy
3.2Å resolution

Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-104536 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Acr-like protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 21.46 KDa
Source organism: Streptococcus phage D1811
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U7VKE8 (Residues: 1-183; Coverage: 100%)
Gene name: D1811_026
CRISPR-associated endonuclease Cas9 1 Chain: C
Molecule details ›
Chain: C
Length: 1121 amino acids
Theoretical weight: 129.7 KDa
Source organism: Streptococcus thermophilus LMD-9
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03LF7 (Residues: 1-1121; Coverage: 100%)
Gene names: STER_0709, cas9-1, csn1
Sequence domains:
tDNA20 Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
sgRNA Chain: D
Molecule details ›
Chain: D
Length: 117 nucleotides
Theoretical weight: 37.44 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-4900
Expression system: Escherichia coli