› 6rjn

X-ray diffraction
2.3Å resolution

Crystal structure of a Fungal Catalase at 2.3 Angstroms

Released:
DOI: 10.2210/pdb6rjn/pdb
PDB entry 6rjn coloured by chain, front view.
PDB entry 6rjn coloured by chain, side view.
PDB entry 6rjn coloured by chain, top view.
Source organism: Komagataella pastoris
Primary publication:
Peroxisomal catalases from the yeasts Pichia pastoris and Kluyveromyces lactis as models for oxidative damage in higher eukaryotes.
Gómez S, Navas-Yuste S, Payne AM, Rivera W, López-Estepa M, Brangbour C, Fullà D, Juanhuix J, Fernández FJ, Vega MC
Free Radic Biol Med 141 279-290 (2019)
PMID: 31238127

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 512 amino acids
Theoretical weight: 58.07 KDa
Source organism: Komagataella pastoris
Expression system: Escherichia coli
UniProt:
  • Canonical: C1PHG1 (Residues: 1-510; Coverage: 100%)
Gene name: CTA1
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
7 bound ligands:
Ligand NDP 4 x NDP
Ligand GOL 17 x GOL
Ligand SO4 2 x SO4
Ligand CL 5 x CL
Ligand K 7 x K
Ligand NA 4 x NA
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21212
Unit cell:
a: 165.943Å b: 173.69Å c: 96.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.142 0.196
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Multi-Faceted Systems Biology Approaches Present a Cellular Landscape of Phenolic Compound Inhibition in Saccharomyces cerevisiae.
Fletcher et al. (2020)
1 more

1 mention without citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)