6rml

X-ray diffraction
2.81Å resolution

Crystal structure of TOPBP1 BRCT0,1,2 in complex with a 53BP1 phosphopeptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-171425 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA topoisomerase 2-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 33.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92547 (Residues: 1-290; Coverage: 19%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
TP53-binding protein 1 Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.76 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q12888 (Residues: 663-677; Coverage: 1%)
Gene name: TP53BP1

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 84.44Å b: 84.44Å c: 225.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.223 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided