› 6rn3

Electron Microscopy
4Å resolution

ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2A

Released:
DOI: 10.2210/pdb6rn3/pdb
PDB entry 6rn3 coloured by chain, front view.
PDB entry 6rn3 coloured by chain, side view.
PDB entry 6rn3 coloured by chain, top view.
Source organisms:
Primary publication:
Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.
Deville C, Franke K, Mogk A, Bukau B, Saibil HR
OpenAccess logo Cell Rep 27 3433-3446.e4 (2019)
PMID: 31216466
Related structures: EMD-4941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167400 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein ClpB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 858 amino acids
Theoretical weight: 95.73 KDa
Source organism: Escherichia coli HVH 50 (4-2593475)
Expression system: Escherichia coli
casein Chain: S
Molecule details ›
Chain: S
Length: 24 amino acids
Theoretical weight: 2.06 KDa
Source organism: Bos taurus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand AGS 9 x AGS
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4Å
Relevant EMDB volumes: EMD-4941
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The molecular principles governing the activity and functional diversity of AAA+ proteins.
Puchades et al. (2020)
8 more

4 mentions without citation

Stairway to translocation: AAA+ motor structures reveal the mechanisms of ATP-dependent substrate translocation.
Gates et al. (2020)
3 more