› 6ro1

X-ray diffraction
3.07Å resolution

X-ray crystal structure of the MTR4 NVL complex

Released:
DOI: 10.2210/pdb6ro1/pdb
PDB entry 6ro1 coloured by chain, front view.
PDB entry 6ro1 coloured by chain, side view.
PDB entry 6ro1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs.
Lingaraju M, Johnsen D, Schlundt A, Langer LM, Basquin J, Sattler M, Heick Jensen T, Falk S, Conti E
Nat Commun 10 3393 (2019)
PMID: 31358741

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127452 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Exosome RNA helicase MTR4 Chain: A
Molecule details ›
Chain: A
Length: 973 amino acids
Theoretical weight: 110.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42285 (Residues: 70-1042; Coverage: 93%)
Gene names: DOB1, KIAA0052, MTR4, MTREX, SKIV2L2
Sequence domains:
Nuclear valosin-containing protein-like Chain: B
Molecule details ›
Chain: B
Length: 55 amino acids
Theoretical weight: 6.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15381 (Residues: 167-216; Coverage: 6%)
Gene names: NVL, NVL2

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand ADP 1 x ADP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P61
Unit cell:
a: 184.365Å b: 184.365Å c: 90.528Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Genetics of human telomere biology disorders.
Revy et al. (2023)
8 more

4 mentions without citation

RNA helicases are hubs that orchestrate exosome-dependent 3'-5' decay.
Weick et al. (2021)
3 more